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CircRNA CRDMO
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circRNA CRDMO Service
Circular RNA, with greater stability attributed to its unique closed-loop structure, is an ideal platform for the development of next-generation gene therapy as an effective alternative to linear mRNA. Geneseed has been focusing on circRNA for more than 10 years and has profound technology accumulation. Geneseed has two patented RNA circularization strategies: Group Ⅰintron self-splicing and T4 Rnl2 method. We have established platforms for sequence design, process development, analytical method development, scale production, QC, raw material preparation, etc., which can provide biopharma with CRDMO services in the whole process from early R&D to commercial production.
Our Serveice
Manufacturing Process from Plasmid to circRNA
Quality Analysis of circRNA
Quality Analysis of circRNA-LNP
Advantages
1. Two independent intellectual property rights, comprehensively matching the needs of the project：circPrecise® by Group I intron self-splicing，circPure® by T4 Rnl2；
2. Unique sequence design approach，circularization efficiency＞90%：using modular translation element screening and secondary structure prediction of IRES and CDS interaction, the translation efficiency is greatly improved；
3. Robust amplification production process，mass production scale up：flexible IVT- circularization scale from μg to g grade for different templates；
4. Various purification strategies，purity＞95%：analyze impurity components and concentration to customize a personalized chromatographic purification strategy；
5. Common LNP encapsulation platform，efficiency＞95%：self-developed liposome screening, platform for different formulations；
6. Multi-dimensional QC platform：develop analytical methods to match the needs of project, and meet the QC evaluation of each stage;
7. Efficient raw material synthesis platform to reduce production costs significantly.
Catalog CircRNA Products
Except for circ-EPO, circ-Trp53, circ-TP53, circ-eGFP, circ-Fluc, circ-mCherry and Natural Circular RNA, 3xFlag labels were inserted to other Catalog CircRNA Products, and anti-Flag antibodies can be used to detect their protein products.
Product Category
Name
Model
Specification
Reporter gene
GSPure® Circ-eGFP
R0701 / R0702 / R0703 / R0704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-Fluc
R0801 / R0802 / R0803 / R0804
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-mCherry
R0901 / R0902 / R0903 / R0904
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-beta gal
R1401 / R1402 / R1403 / R1404
100 μg / 500 μg / 1 mg / 5 mg
Tumor antigen
GSPure® Circ-OVA
R1501 / R1502 / R1503 / R1504
100 μg / 500 μg / 1 mg / 5 mg
Anti-tumor
GSPure® Circ-TP53
R1701 / R1702 / R1703 / R1704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-Trp53
R2501 / R2502 / R2503 / R2504
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-PTEN-L (homo)
R1001 / R1002 / R1003 / R1004
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-PTEN-L (mmu)
R1101 / R1102 / R1103 / R1104
100 μg / 500 μg / 1 mg / 5 mg
Growth factor
GSPure® Circ-VEGFA165 (homo)
R1201 / R1202 / R1203 / R1204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-VEGFA165 (mmu)
R1301 / R1302 / R1303 / R1304
100 μg / 500 μg / 1 mg / 5 mg
immunotherapy
GSPure® Circ-IL2 (homo)
R1901 / R1902 / R1903 / R1904
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-IL2 (mmu)
R3201 / R3202 / R3203 / R3204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-IL12a
R3301 / R3302 / R3303 / R3304
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-IL12A
R3401 / R3402 / R3403 / R3404
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-IL12B
R3501 / R3502 / R3503 / R3504
100 μg / 500 μg / 1 mg / 5 mg
Gene editing
GSPure® Circ-Cas9
R2001 / R2002 / R2003 / R2004
100 μg / 500 μg / 1 mg / 5 mg
Protein/
Antibody replacement
GSPure® Circ-EPO (homo)
R2101 / R2102 / R2103 / R2104
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-EPO (mmu)
R2301 / R2302 / R2303 / R2304
100 μg / 500 μg / 1 mg / 5 mg
iPSC reprogramming
GSPure® Circ-SOX2 (homo)
R2201 / R2202 / R2203 / R2204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-SOX2 (mmu)
R2401 / R2402 / R2403 / R2404
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-OCT4 (homo)
R2601 / R2602 / R2603 / R2604
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-OCT4 (mmu)
R2701 / R2702 / R2703 / R2704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-KLF4 (homo)
R2801 / R2802 / R2803 / R2804
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-KLF4 (mmu)
R2901 / R2902 / R2903 / R2904
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-C-Myc (homo)
R3001 / R3002 / R3003 / R3004
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-C-Myc (mmu)
R3101 / R3102 / R3103 / R3104
100 μg / 500 μg / 1 mg / 5 mg
Anti-aging
GSPure® Circ-TERT
R3601 / R3602 / R3603 / R3604
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-Tert
R3701 / R3702 / R3703 / R3704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FGF21 (homo)
R3801 / R3802 / R3803 / R3804
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FGF21 (mmu)
R3901 / R3902 / R3903 / R3904
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-αKlotho (homo)
R4001 / R4002 / R4003 / R4004
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-αKlotho (mmu)
R4101 / R4102 / R4103 / R4104
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-sTGFβR2 (homo)
R4201 / R4202 / R4203 / R4204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-sTGFβR2 (mmu)
R4301 / R4302 / R4303 / R4304
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FGF23 (homo)
R4401 / R4402 / R4403 / R4404
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FGF23 (mmu)
R4501 / R4502 / R4503 / R4504
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FGF17 (homo)
R4601 / R4602 / R4603 / R4604
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FGF17 (mmu)
R4701 / R4702 / R4703 / R4704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-SOX8 (homo)
R4801 / R4802 / R4803 / R4804
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-SOX8 (mmu)
R4901 / R4902 / R4903 / R4904
100 μg / 500 μg / 1 mg / 5 mg
Neurotrophic factor
GSPure® Circ-NGF (homo)
R5001 / R5002 / R5003 / R5004
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NGF (mmu)
R5101 / R5102 / R5103 / R5104
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-BDNF (homo)
R5201 / R5202 / R5203 / R5204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-BDNF (mmu)
R5301 / R5302 / R5303 / R5304
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-GDNF (homo)
R5401 / R5402 / R5403 / R5404
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-GDNF (mmu)
R5501 / R5502 / R5503 / R5504
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NT3 (homo)
R5601 / R5602 / R5603 / R5604
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NT3 (mmu)
R5701 / R5702 / R5703 / R5704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NT4 (homo)
R5801 / R5802 / R5803 / R5804
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NT4 (mmu)
R5901 / R5902 / R5903 / R5904
100 μg / 500 μg / 1 mg / 5 mg
Nerve regeneration
transforming factor
GSPure® Circ-NeuroD1 (homo)
R6001 / R6002 / R6003 / R6004
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NeuroD1 (mmu)
R6101 / R6102 / R6103 / R6104
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NeuroD2 (homo)
R6201 / R6202 / R6203 / R6204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NeuroD2 (mmu)
R6301 / R6302 / R6303 / R6304
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NeuroD6 (homo)
R6401 / R6402 / R6403 / R6404
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NeuroD6 (mmu)
R6501 / R6502 / R6503 / R6504
100 μg / 500 μg / 1 mg / 5 mg
Natural circular RNA
GSPure® Circ-PVT1 (homo)
R6601 / R6602 / R6603 / R6604
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-PVT1 (mmu)
R6701 / R6702 / R6703 / R6704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FOXO3 (homo)
R6801 / R6802 / R6803 / R6804
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-FOXO3 (mmu)
R6901 / R6902 / R6903 / R6904
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-HIPK3
R7001 / R7002 / R7003 / R7004
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-ZNF609
R7101 / R7102 / R7103 / R7104
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-ZFR
R7201 / R7202 / R7203 / R7204
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-SMARCA5
R7301 / R7302 / R7303 / R7304
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-PRKCI
R7401 / R7402 / R7403 / R7404
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-NRIP1
R7501 / R7502 / R7503 / R7504
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-ABCB10
R7601 / R7602 / R7603 / R7604
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-CDYL
R7701 / R7702 / R7703 / R7704
100 μg / 500 μg / 1 mg / 5 mg
GSPure® Circ-Mbl
R7801 / R7802 / R7803 / R7804
100 μg / 500 μg / 1 mg / 5 mg
High integrity
Figure 1: GSPure® Circ-eGFP (left) and GSPure® Circ-mCherry (right) integrity (CE) > 95% after purification
High purity
Figure 2: GSPure® Circ-eGFP (left) and GSPure® Circ-Fluc (right) purity (HPLC) > 95% after purification
High quality
Fig 3: Fluorescence detection results after GSPure®Circ-eGFP transfected 293T cells, magnification: 100×.
Fig 4: Fluorescence detection results after GSPure® Circ-mCherry transfected 293T cells, magnification: 100×.
Fig 5: Results of luciferase detection after GSPure® Circ-Fluc transfection of 293T cells.
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